broth recovered after the Fermentation process is composed of a mix of
substances such as unutilized nutrients, microorganisms, production
proteins, fermentation additives and water. The end use of the protein
of interest requires it to be at a specific purity depending upon the
application such as Food Processing, Animal Feed etc. Hence an optimum
Downstream Processing technique is essential to recover the products of
interest from the large fermentation broth.
this technique may seem to be pure process oriented, the reality is
more than it meets the eye. Downstream process is among the major
factors which is directly related to the cost of the finished product.
An efficient process allows for maximum extraction of the target
protein from the fermentation broth while maintaining its activity
specifications. Moreover perfecting a process in the laboratory and
actually performing it in large scale gives quite different results. It
is imperative to maintain the same efficiency standards during bulk
processing as previously conducted in laboratory scales, else the final
results may vary. Another factor towards achieving efficiency
developing a process which works constantly once initiated, saving
valuable overhead costs and energy resources.
Industrial Biotechnology, especially in the manufacture of Industrial
Enzymes, the processing steps for recovery of enzymes from the
fermentation broth is applied in a systematic and continous order. They
slightly differ depending upon the Fermentation type (Solid Substrate
or Submerged) but basically have the same principles. The steps involve
removal of insolubles by pre-filtration, cell disruption if the product
is intra-cellular, cell separation and purification &
of the target protein prior to formulation. There is one additional
step in Solid Substrate fermentation, i.e Extraction of
the Enzymes from the growth substrate or biomass.
techniques such as Centrifugation, Homogenization &
Ultrasonification, Precipitation, Membrane filtration such as
Microfiltration & Ultrafiltration etc are employed for the
an efficient downstream processing technique and executing it to
perfection is the ardent task which our bioprocess engineers undertake
at Noor Creations.